Tissue Dissociation Steps [VIDEO]
Flow cytometry is most often used to study cells from the immune system. These cells isolated often from blood in the peripheral circulation, but can also be harvested from tissues. Cells found in tissues or organs are usually tightly attached to these sites, and this is a fundamental property of these cells that allows them to surveil the immune system and launch responses. However, additional cell preparation steps must be used in order to dissociate these cells from the tissue so they can be used for flow cytometry.
In this video, we demonstrate a protocol for dissociating Rat Spleens into a single cell suspension and downstream flow cytometry assays.