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Single-Cell Proteomic Services

FlowMetric is proud to be extending our service offering to include comprehensive, single-cell functional proteomics with the IsoLight Proteomics Hub from IsoPlexis. This multiplexed system offers discovery of biomarkers from the secretome and intracellular proteome, providing a means of describing each immune cell by its true functional biology.

The IsoLight system can support different applications, across many fields of clinical research, including immuno-oncology, autoimmune disorders, vaccine and drug responses, and infectious disease. With more than 70 publications and 50 highly correlative clinical datasets, this innovative platform is providing unparalleled predictive data across diverse areas of medicine.

IsoCode Technology Provides Single-Cell, Highly Multiplexed Analysis.

At the heart of the IsoCode technology is the chips that mediate the capturing of thousands of single cells into autonomous chambers, enabling the biological function of each of these cells to be measured in isolation. Optimized panels are available to resolve the cellular secretome, along with intracellular proteome and metabolome profiles.

IsoCode Barcoded Chip Design: Single-Cell Chamber Equipped with Multiplex ELISA Cytokine Capture

Family of IsoCode Chips: Single-Cell Functional Secretome Analysis

Single-Cell Adaptive Immune T-cells

Applications-
Predictive Pre-clinical Potency
Pre-clinical Persistence Modeling
Protective Immune Monitoring
Stratification of Patients
T-cell Functional Profiling
Biomarkers-
Granzyme B, IFN-γ, MIP-1α, Perforin, TNF-α, TNF-β, GM-CSF, IL-2, IL-5, IL-7, IL-8, IL-22, IL-15, IL-21, CCL11, IP-10, MIP-1β, RANTES, IL-4, IL-10, IL-22, TGFβ1, sCD137, sCD40L, IL-1β, IL-6, IL-17A, IL-17F, MCP-1, MCP-4

Single-Cell Adaptive Innate Immune NK, Monocytes, Macrophages, Fibroblast Cells

Applications-
Pre-clinical Myeloid Impact
Prognostic Immune Suppression
Profiling of Early Response Function
Predictive for Neuroinflammation
Innate Cell Functional Profiling
Biomarkers-
IFN-γ, MIP-1α, TNF-α, TNF-β, GM-CSF, IL-5, L-8, IL-9, IL-15, IL-18, TGF-α, CCL11, IP-10, MIP-1β, RANTES, BCA-1, IL-10, IL-13, IL-22, sCD40L, IL-1β, IL-6, IL-22, IL-17A, IL-17F, MCP-1, MCP-4, MIF, EGF, PDGF-BB, VEGF

Single-Cell Inflammation T-cells

Applications-
Prognostic of autoimmune responses
Pre-clinical Immune Suppression
Biomarkers-
GM-CSF, IFN-γ, IL-2, IL-22, TNF-α, TNF-β , IL-4, IL-5, IL-7, IL-9, IL-13, CCL11, IL-8, IP-10, MCP-1, MCP-4, MIP-α, MIP-1β, RANTES, IL-10, IL-15, IL-22, TGF-β1, IL-1β, IL-6, IL-17A, IL-17F, IL-21, Granzyme B, Perforin, sCD40L, sCD137

Single-Cell Inflammation T-cells

Applications-
Predictive Resistance
Kinase Modeling
Patient Stratification
Overcoming Inhibitor Resistance
Biomarkers-
GM-CSF, IFN-γ, IL-2, IL-22, TNF-α, TNF-β, IL-4, IL-5, IL-7, IL-9, IL-13, CCL11, IL-8, IP-10, MCP-1, MCP-4, MIP-α, MIP-1β, RANTES, IL-10, IL-15, IL-22, TGF-β1, IL-1β, IL-6, IL-17A, IL-17F, IL-21, Granzyme B, Perforin, sCD40L, sCD137

The CodePlex solution uses ultra-low sample volumes, to provide big data resolution.

Isoplexis’ CodePlex solution provides a streamlined approach to multiplexed bulk cytokine analytics. With more than 20 parameters measured from one 11μL sample volume, panels are optimized for specific research applications to deliver unprecedented insights into functional drivers within biological systems.

CodePlex Barcoded Chip Design: Small Bulk Sample Multiplex ELISA Cytokine Capture

Family of CodePlex Secretome Chips

Human Adaptive Immune

Applications-
Predictive Pre-clinical Potency
Bioprocess Optimization
Protective Immune Monitoring
Biomarkers-
IL-17A, TGF-b1, MIP-1a, IL-9, MIP-1b, IL-6, IL-7, IL-8, IFN-γ, IP-10, GM-CSF, IL-4, IL-5, IL-10, TNF-α, MCP-1, IL-13, IL-2, Perforin, sCD40L, sCD137, TNF-b, Granzyme B, IL-15

Human Cytokine Storm

Applications-
Cytokine Storm During Infection
Cytokine Storm During Cell Therapy
Immune-related Adverse Effect Screening
Biomarkers-
IL-1b, IL-2, IL-4, IL-6, IL-7, IL-10, IL-12, IL-13, IL-17, IL-10, MCP-1, GM-CSF, MIP-1a, MIP-1b, TNF-α, IFN-γ

Human Innate Immune

Applications-
Immuno Suppression
Pre-clinical Myeloid Impact
Neuroinflammation
Biomarkers-
IL-17, TGF-B1, MIP-1a, MIP-1b, IL-6, IL-7, IL-8, IFN-γ, IP-10, GM-CSF, IL-4, IL-5, IL-10, TNF-α, MCP-1, IL-2, Perforin, sCD40L, sCD137, TNF-b, Granzyme B, IL-15, PDGF-BB

Cancer Signaling

Applications-
Immuno Profiling
Therapeutic Optimization
Patient Stratification
Biomarkers-
IL-6, IL-7, IFN-γ, IL-4, IL-5, IL-10, TNF-α, MCP-1, IL-13, IL-2, EGF, PDGF-BB, VEGF, RANTES (MPN), MIF, FGF, HGF, IL-1a, IL-1b, IL-12

Stem Cell Signaling

Applications-
Immuno Profiling
Regulation of Plasticity and Pluripotency
Biomarkers-
IL-17A, TGF-b1, MIP-1a, MIP-1b, IL-6, IL-8, IFN-γ, GM-CSF, IL-4, IL-10, TNF-α, MCP-1, IL-2, IL-15, RANTES (MPN), IL-1a, IL-1b, IL-12, CCL@, CXCL5 (MPN)

Data Analytics

As with flow cytometry, the IsoLight system produces large datasets. FlowMetric’s expertise in big data analytics will ensure you get the most out of your clinical samples. As with our flow cytometry services, we can provide multiple analytical readouts and reportables, in whatever format your study requires.

tSNE and heatmap analysis of your data sets to uncover critical subpopulations in your samples of interest. Our analysis can also determine polyfunctionality and dominant functional groups within your samples of interest and identify the biological drivers behind responses.

Single Cell Poly Functional Strength Index represents a novel and revealing metric for measuring the potency of different immune cell types.

For more information on the IsoLight system and how FlowMetric can support your functional proteomics research initiatives, please contact us today and speak with one of our technical support team members.

 

Speak With A Proteomics Expert