Complement-dependent cytotoxicity (CDC)
Complement-dependent cytotoxicity (CDC) is the mechanism by which cells covered by antibodies activate the complement pathway, also known as the membrane attack complex or MAC, opening holes in the membrane of the cells, and ultimately killing cells. With CDC assays we can assess antibodies with this capability to be potentially used as therapeutics. Complement-dependent cytotoxicity assays require the use of a cell line to incubate with an antibody, the measurement of cytotoxicity is carried out using Flow Cytometry.
At FlowMetric we have optimized Complement-dependent cytotoxicity assays to evaluate antibodies that can be used as therapeutics and we can provide quantitative analysis to support your research.