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Flow Cytometry Blog

Do’s And Don’ts Of Clinical Flow Cytometry

Posted on: October 16, 2019

We’ve given you advice about what to consider when planning clinical flow cytometry experiments. Now check out these ‘do’s and don’ts’ to get the most out of your next clinical flow experiment.

Do - Know What Types Of Cells You Want To Identify.

Some cells are very robust and are easy to identify in peripheral blood samples. Other cells die if you shake the tube the wrong way or they behave as if they have their own agenda. Take the time to do pilot experiments with samples that are not precious so you can work out a protocol that is robust and precise enough to identify your cells of interest in your valuable clinical samples.

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Insights Begin with a Single Cell: Detection of Phosphorylated Proteins Using Flow Cytometry

Posted on: October 09, 2019

Flow cytometry is a powerful technique that can analyze properties of individual cells and measure millions of cells at a time. Single cell analysis typically includes measurements of surface and intracellular proteins, but protein modifications can also be detected by flow cytometry. Phosphoflow assays measure phosphorylated proteins and offers several advantages over traditional lysate-based phosphorylation detection assays.

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Mind the Mechanism - Using Flow Cytometry to Determine Mechanisms of Action for Therapeutic Antibodies

Posted on: October 02, 2019

Immunotherapy research is a rapidly expanding field in which dozens of monoclonal antibodies are being developed to treat cancer and autoimmune diseases. The mechanism of action (MOA) used by an antibody to mediate a therapeutic response must be defined in order for a candidate antibody to advance down the preclinical development pipeline. Defining the MOA is necessary to fulfilling regulatory requirements for antibodies used in clinical trials and also critical to understanding if the antibody may cause any detrimental side effects.

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PrimeFlow – Linking RNA and Protein Expression in a Single Cell by Flow Cytometry

Posted on: September 25, 2019

Flow cytometry is a powerful way to characterize protein expression at the single cell level, but new techniques are now using flow cytometry to measure RNA expression within individual cells. The PrimeFlow RNA assay is a method for measuring RNA in cells that is based on the fluorescent in situ hybridization technique (FISH) in which sequence-specific oligonucleotide probes are used together with DNA and fluorescent labels to specifically detect and amplify the signal for a specific RNA target. Consider these key features of PrimeFlow as you decide if it would be a valuable addition to your cell analysis toolkit.

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Making Hybridomas Happen - 5 Tips For Creating and Optimizing Your Selection of Hybridoma Clones

Posted on: September 18, 2019

Hybridoma technology has revolutionized how antibodies are created in the laboratory and has been essential to the development of therapeutic antibodies for treatment of cancer, autoimmune disorders and the development of innovative diagnostics. Hybridomas are cell fusions of splenocytes collected from mice immunized with an antigen of interest and immortalized myeloma cells. A single hybridoma multiplies into cell clones and produces a monoclonal antibody against an epitope of the immunizing antigen, and this secreted antibody can be collected and used for a wide variety of applications. You can make hybridomas in your own laboratory with an appropriate animal protocol in place, or you can work with an experienced contract research organization to help you create and develop a panel of hybridomas for your next project.

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Computational Cytometry | Flow Cytometry Data Analysis in the Era of Quantitative Data Science

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