Flow cytometry analysis is a critical technique for guiding preclinical drug development. Flow cytometry data can provide mechanistic insights and define potential biomarkers that correspond to clinical outcomes. But getting the most out of your preclinical flow cytometry analysis may hinge on how your cell samples are handled, specifically whether you are working with fresh cells or fixed cells.
Flow Cytometry Blog
What is flow cytometry? Flow cytometry is a semi-quantitative technique that allows you to analyze the frequency and other properties of cells stained with specific fluorochrome-conjugated antibodies. Flow cytometry is most frequently used to monitor the immune response due to the fact that the frequency and functionality of different immune cell subsets can be measured concurrently. Flow cytometers are the instrument at the center of this technique and have evolved over the past few decades to become an essential instrument for biomedical research.
Flow cytometry assays can only deliver reliable, high quality data if the cells used in the assays are viable and handled properly. Immune cells, including lymphocytes and dendritic cells, can be isolated from whole blood using density gradient centrifugation protocols. These peripheral blood mononuclear cells (PBMCs) can be used as fresh cells for flow cytometry or can be frozen for later use. Consider the pros and cons of using each type of cell preparation when you are planning your next flow cytometry assay.
Intracellular staining (ICS) assays are one of the most commonly used flow cytometry assays in basic and clinical research. These customizable assays allow users to measure various cytokine responses in different immune cell types. But why go through all this effort when ELISA or bead-based assays can also measure cytokines? Check out these three features of ICS assays that make them stand out from similar cytokine assays.
Flow cytometry is a powerful tool for surveying the cellular landscape during preclinical development of drugs and biologics. But flow cytometry can go beyond immunophenotyping to actual functional measurements that can contribute to understanding the true potential of a therapeutic candidate. To make the most of your flow cytometry studies, consider these other assays as you plan the next phase of preclinical development.
Posted in Preclinical Development