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Flow Cytometry Blog

PrimeFlow – Linking RNA and Protein Expression in a Single Cell by Flow Cytometry

Posted on: September 25, 2019

Flow cytometry is a powerful way to characterize protein expression at the single cell level, but new techniques are now using flow cytometry to measure RNA expression within individual cells. The PrimeFlow RNA assay is a method for measuring RNA in cells that is based on the fluorescent in situ hybridization technique (FISH) in which sequence-specific oligonucleotide probes are used together with DNA and fluorescent labels to specifically detect and amplify the signal for a specific RNA target. Consider these key features of PrimeFlow as you decide if it would be a valuable addition to your cell analysis toolkit.

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Sorting out the Details: Three Factors to Consider for Cell Sorting by Flow Cytometry

Posted on: September 11, 2019

Fluorescent-Activated Cell Sorting (FACS) is a flow cytometry-based technique in which cells are stained with fluorescently labeled antibodies and sorted based on pre-defined staining parameters that are specific to different cell types. FACS users must consider multiple factors when designing and running a FACS experiment. Consider these three factors as you plan and carry out your next FACS experiment.

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Three Reasons to Partner with a Specialty CRO

Posted on: August 07, 2019

When it comes to handing over your precious research samples to a contract research organization (CRO), how do you know you are outsourcing to the right company? Larger CROs seem to offer every type of analysis available, from genomics to histopathology. But specialty CROs may be a better option, especially if you need sophisticated techniques and data analysis.

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Where did my cells go? On summer vacation???

Posted on: July 24, 2019

Have you ever started a flow cytometry run and you cannot see any cells? This can be an alarming feeling, but some straightforward troubleshooting can usually help you find your missing cells. Check out these three questions to tackle common issues associated with disappearing cells.

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Receptor Occupancy in the Clinic

Posted on: July 10, 2019

Immunotherapeutic molecules currently being used in the clinic are powerful immune modulators, but their effectiveness can be inconsistent between patients. Clinicians and scientists use different assays to evaluate why immunotherapies fail in the clinic. The flow cytometry-based receptor occupancy (RO) assay is a critical tool for evaluating the effectiveness of immunotherapies in the clinic. Here are three features of flow cytometry-based RO assays that give them clinical value.

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Computational Cytometry | Flow Cytometry Data Analysis in the Era of Quantitative Data Science
 

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