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Flow Cytometry Blog

Sorting out the Details: Three Factors to Consider for Cell Sorting by Flow Cytometry

Posted on: September 11, 2019

Fluorescent-Activated Cell Sorting (FACS) is a flow cytometry-based technique in which cells are stained with fluorescently labeled antibodies and sorted based on pre-defined staining parameters that are specific to different cell types. FACS users must consider multiple factors when designing and running a FACS experiment. Consider these three factors as you plan and carry out your next FACS experiment.

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Measuring Memory - Evaluating Memory T Cells In A Clinical Setting

Posted on: September 04, 2019

Memory is a characteristic of the immune system that provides humans and other vertebrates with long term protection against infectious diseases and other “non-self” antigens such as those associated with tumor cells. In the context of T cells, memory responses occur when a naive T cell encounters an antigen bound to a major histocompatibility complex molecule and is activated to undergo differentiation into an effector cell or a memory cell. Memory T cell populations can persist in the body for months to years and can be stimulated to respond specifically and rapidly to a foreign antigen upon re-exposure.

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Cyno versus Rhesus NHPs - Things to think about when choosing non-human primate samples in your Flow Cytometry Studies

Posted on: August 28, 2019

Non-human primates (NHPs) are a valuable research tool, especially in the development of vaccines and treatments for infectious diseases like HIV[1]. Although biomedical researchers have made significant advances in reducing the number of NHPs used in research, some preclinical experiments can only be carried out in these unique animal models.

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Outstanding Omics: Genomics, Proteomics, and Flow Cytometry

Posted on: August 22, 2019

We live in an era of data-driven science in which we can sequence the genomes of individual cells and measure the full complement of proteins contained within. How do we acquire and measure such data? The vast world of the “-omics,” including genomics and proteomics, has become accessible to research scientists in basic and clinical research sectors because of the rise of new tools and techniques that have improved the speed, accuracy, and affordability of the methods. Flow cytometry has been a critical technique in this revolution as it allows for the detection and sorting of individual cells. Check out the main features of each of these “-omics” fields and how they complement your current research projects.

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Flow Cytometry for Cell Cycle Analysis

Posted on: August 14, 2019

The cell cycle is a defining characteristic of living organisms. In multicellular organisms, including humans, the cell cycle governs the duplication of cells in a manner that assures accurate DNA replication and cell division. Cell cycle regulation is considered such an essential element of biology that Leland H. Hartwell, R. Timothy Hunt and Paul M. Nurse were recognized with the 2001 Nobel Prize in Physiology for their discoveries of "key regulators of the cell cycle."

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Computational Cytometry | Flow Cytometry Data Analysis in the Era of Quantitative Data Science

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