Immunotherapeutic molecules currently being used in the clinic are powerful immune modulators, but their effectiveness can be inconsistent between patients. Clinicians and scientists use different assays to evaluate why immunotherapies fail in the clinic. The flow cytometry-based receptor occupancy (RO) assay is a critical tool for evaluating the effectiveness of immunotherapies in the clinic. Here are three features of flow cytometry-based RO assays that give them clinical value.
Flow Cytometry Blog
Flow cytometry is becoming a standard tool in clinical and translational immuno-oncology (IO) research because it allows researchers to monitor dynamic changes in the immune system and can be customized to study different cancers. Flow cytometry-based immunophenotyping assays using whole blood are especially well suited to IO research because various cells subsets can be profiled in a single assay including naive CD4+ and CD8+ T cells, central and effector memory T cells, regulatory T cells, B cell subsets, NK cells, monocytes/macrophages, and dendritic cell subsets. These assays can be used for evaluating all types of IO therapies including checkpoint inhibitors, adoptive cell therapies, and cancer vaccines.
Toxicology screening is essential to any preclinical study, and flow cytometry-based toxicology assays are a fast and practical approach. Different animal models are used for toxicology studies, including rodents, dogs and non-human primates. One commonly used technique is the micronucleated erythrocyte endpoint assay, which measures DNA damage induced by exposure to experimental drugs or biologics. This method has been adapted into a validated flow cytometry-based assay and can use erythrocytes from different species. Consider these factors when selecting the best toxicology screening method.
Posted in Toxicology & Safety
High quality flow cytometry results start with proper collection, processing and storage of cell specimens. Several factors determine which type of test tube to use for sample collection, including the cell type you are studying, and your preservation and shipping needs.
What is the Technology?
Flow Cytometry and Mass Spectrometry are widely used analytical tools in cancer research. Flow Cytometry (FACS or Fluorescence Activated Cell Sorting) has been well established for cell counting, cell sorting and biomarker discovery. Its ability to utilize 14+ colors makes it the technique-of-choice for isolation of many cell subtypes especially rare cells such as stem cells. Mass Spectrometry is unparalleled in its ability to selectively detect and quantify target proteins at the molecular level. By combining these two powerful techniques, it is possible to isolate highly selective cellular populations from biological fluids and quantify the cell’s specific proteins.
Posted in Preclinical Development