Cell sorting is not limited to studying cells derived from blood or tissue samples, but it is also a valuable too for characterizing cell lines. Cell lines are critical to basic research studies as well as biopharmaceutical product development and production. Consider these cell sorting applications for your cell line needs.
Flow Cytometry Blog
Immune cell infiltration is essential for anti-tumor responses because immune cells must be able to be in close proximity to tumor cells for these responses to be effective. Flow cytometry is a flexible and powerful tool for detecting and monitoring the presence of infiltrating immune cells, or TIL, and can be customized to detect any immune cell subset.
Consider these questions as you think about assessing infiltrating immune cells with flow cytometry in your Immuno-Oncology (IO) research.
Regulatory T cells (Tregs) are an important cell subpopulation because they can modulate immune responses that target self-antigens but are also associated with poor outcomes in cancer patients. Tregs are found in peripheral blood and are defined by both surface and intracellular markers, but there is still some debate as to the optimal staining panel for monitoring Tregs, particularly during immunotherapeutic interventions for cancer therapy and autoimmune diseases.
Flow cytometry is a powerful tool because it allows users to analyze the characteristics of millions of cells with relative speed and precision. A single cell suspension of fluorescently labeled sample travels through the cytometer for excitation by lasers, and the emitted light photons are measured by different detectors. Having a single cell suspension is essential to measuring cell fluorescence accurately, and many types of cell or tissue samples must be specially processed to make this suspension. Two different methods can be used for single cell suspensions: mechanical dissociation of tissue or enzymatic dissociation of tissue. This processing step is typically carried out before cells are stained and both methods have benefits and caveats.
Flow cytometry analysis is a critical technique for guiding preclinical drug development. Flow cytometry data can provide mechanistic insights and define potential biomarkers that correspond to clinical outcomes. But getting the most out of your preclinical flow cytometry analysis may hinge on how your cell samples are handled, specifically whether you are working with fresh cells or fixed cells.