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Flow Cytometry Blog

FlowMetric’s Response to COVID-19

Posted on: March 30, 2020

Dear Colleagues,

FlowMetric’s labs and offices are fully operational and we continue to support our sponsors, their projects, and patients. Under direction from the Governor’s office, FlowMetric is considered an essential business and thus we are doing everything we can to retain strong business continuity while keeping our employees and our community safe. 

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Myeloid-Derived Suppressor Cells – Using Flow Cytometry to Understand these Immune Suppressive Heavyweights in Cancer

Posted on: December 18, 2019

Myeloid-derived Suppressor Cells (MDSCs) have come to be known as key regulators of the immune system, particularly with their role in promoting tumor progression. At least two major subsets of MDSCs have been defined in mouse models based on phenotypic features - monocytic-MDSCS (M-MDSCs) and polymorphonuclear-MDSCs (PMN-MDSC). Characterization of human MDSCs in cancer patients has revealed a more complicated picture of MDSC subsets. Flow cytometry has been essential characterizing MDSCs and understanding their role in different cancers. Here are three ways flow cytometry has been invaluable to understanding MDSCs.

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Keep Calm and Contract Your Cytometry

Posted on: December 11, 2019

Top 5 Things to Look for in a Flow Cytometry CRO

There comes a moment when the flow cytometry work can be too much. Sometimes it’s the big “make-or-break” experiment that has to go just right, or sometimes it’s the monthly batches of clinical trial samples that have to be handled, on a Saturday, with swift, deft hands, and you’re the only one qualified for the job. That’s when you either break down in tears of exhaustion and frustration, or you consider finding some help. Now most investigators aren’t in a position to hire and train new staff for cytometry work, but there is high quality and reliable alternative - A contract research organization (CRO).

A CRO can carry out your flow cytometry experiments, to you exact specifications, and meet your needs for a single experiment or a multi-year clinical trial. CROs specializing in flow cytometry may be your best option to guarantee high quality and reliable results.

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The Three P’s of Ploidy – Understanding Ploidy and Flow Cytometry

Posted on: December 05, 2019

Ploidy is defined as the number of complete chromosome sets in a cell’s nucleus. In animal cells, chromosomes are typically found in pairs and cells are considered diploid. In contrast, plant cells can tolerate higher levels of genome duplication and can exist in various states of polyploidy. Understanding and characterizing ploidy in diverse organisms, from bacteria to mammals, has been greatly facilitated by flow cytometry-based ploidy measurements. Consider these three P’s of ploidy as you decide if ploidy analysis could be a valuable addition to a current or upcoming study.

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To Validate or Not Validate... That Is The Assay Validation Question

Posted on: November 27, 2019

Many scientists performing preclinical and clinical research hit a point when they need to have an assay validated. You may have painstakingly developed and perfected a particular assay, but now you must put it through the rigors of validation for it to be considered a “validated assay.” The basic principles of assay validation were described in an earlier blog post, but how do you know you if you need an assay validated? Use these questions as a guide to help you figure out your validation situation and get a little less vexed about validation.

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