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Flow Cytometry Blog

To Fix or Not to Fix – How to Handle Clinical Samples for Flow Cytometry

Posted on: December 12, 2018

Most flow cytometry samples are processed, stained and then run on a flow cytometer, and these protocols may take a few hours but can also span the greater part of a day. Protocols take even longer when many samples needed to be handled, which is often the case with clinical samples. How do you handle staining and running large numbers of clinical samples while also trying to pull an all-nighter? In some cases, a fixative can be added to stained cells so they can be preserved in their stained state, refrigerated, and run on a flow cytometer at a later time. Consider these factors to help you determine if cell fixation is a good option.

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Flow Cytometry Beyond Basic Research: Best Practices In GLP For Flow Cytometry Assays

Posted on: December 05, 2018

Flow cytometry assays are important for preclinical and clinical research, however, it is vital to understand the level of compliance required for the stage of research you are completing.  Flow Cytometry assays completed for toxicology and safety assessments are required to be in compliance of Good Laboratory Practices (GLP), on the other hand, basic research or discovery/exploratory studies can be non-GLP.  GLP refers to a set of standards for laboratory studies to be planned, performed, monitored, reported, and archived. Preclinical and clinical studies must be GLP-compliant in order to be submitted for review by regulatory agencies like the FDA. Consider these three points if you find yourself in need of a GLP-compliant flow cytometry assay. 

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Fast and Furious Flow Cytometry: HTS Assays

Posted on: November 28, 2018

Flow cytometry is becoming an increasingly popular tool for high-throughput screening (HTS) assays for identifying potential new drugs or biologics. Most users are acquainted with flow cytometers that use individual tubes for sample collection, but flow cytometers can be equipped with HTS attachments for automated sample collection for multi-well plates. Check out these benefits of using flow cytometry for HTS assays.

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The Many Colors of Immunophenotyping

Posted on: November 21, 2018

Not too long ago, four-color flow cytometers were widely used for immunophenotyping and provided reliable and cutting edge data. Now, flow cytometry staining panels can include up to 50 colors and provide you with the ability to immunophenotype multiple cell subsets at once. These highly complex panels are attractive because so much data can be gathered from a single experiment, but the sheer size of the staining panel can feel daunting to the most experienced flow cytometry user.

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T Cell Exhaustion – Using Flow Cytometry to Monitor this Immuno-Oncology Impediment

Posted on: November 07, 2018

T cells are well known for their roles in combating cancer and infection, but chronic exposure to antigens and inflammation can cause T cells to enter a state of “exhaustion[1].” Exhausted T cells lose critical effector functions including cytokine production, the ability to proliferate and memory T cell differentiation is also compromised. Exhausted T cells also express inhibitory receptors and become unresponsive to IL-7 and/or IL-15-driven self-renewal. This progression toward T cell exhaustion results in diminished control of chronic infection or cancer. Exhaustion can occur in both CD4+ and CD8+ T cell populations and the phenotypes of these subsets is somewhat heterogeneous. Nonetheless, T cell exhaustion is reversible and various immuno-oncology interventions have been examined or are currently being evaluated in order to improve outcomes in cancer and chronic infection[2].

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