High quality flow cytometry results start with proper collection, processing and storage of cell specimens. Several factors determine which type of test tube to use for sample collection, including the cell type you are studying, and your preservation and shipping needs.
Flow Cytometry Blog
What is the Technology?
Flow Cytometry and Mass Spectrometry are widely used analytical tools in cancer research. Flow Cytometry (FACS or Fluorescence Activated Cell Sorting) has been well established for cell counting, cell sorting and biomarker discovery. Its ability to utilize 14+ colors makes it the technique-of-choice for isolation of many cell subtypes especially rare cells such as stem cells. Mass Spectrometry is unparalleled in its ability to selectively detect and quantify target proteins at the molecular level. By combining these two powerful techniques, it is possible to isolate highly selective cellular populations from biological fluids and quantify the cell’s specific proteins.
Posted in Preclinical Development
Flow cytometry has moved into the era of “big data,” and data plots have also shifted from scatter plots and histograms toward visualization methods that can handle this complex data. This era of computational cytometry has ushered in the use of T-distributed Stochastic Neighbor Embedding (t-SNE) data analysis, a machine learning algorithm applied to complex flow cytometry data. Check out these three reasons why t-SNE data analysis is a valuable data visualization tool for flow cytometry.
Flow cytometry (FC) is a sensitive and highly customizable technique that can be used in both preclinical and clinical studies. How do you ensure that flow cytometry data is reliable, consistent and can meet the standards required for regulatory requirements or clinical trials? Implementing quality control (QC) procedures is a widely accepted strategy to guarantee that your flow cytometry data is handled in a rigorous and consistent manner and can be used for regulatory compliance and clinical trial evaluations.
Posted in Flow Cytometry
Cell sorting is not limited to studying cells derived from blood or tissue samples, but it is also a valuable too for characterizing cell lines. Cell lines are critical to basic research studies as well as biopharmaceutical product development and production. Consider these cell sorting applications for your cell line needs.