We walked you through the basics of achieving your optimal panel design in the “Know your Flow” series, but you probably have more questions as you refine your panel or stumble upon some of the technical nuances of staining cells.
Check out these tips for a successful flow cytometry run.Read the full article »
Flow cytometry analysis only provides usable data if you analyze living cells. In both preclinical and clinical studies, including a live/dead stain in flow cytometry panels is essential for discriminating live from dead cells in order to analyze only live cells. Live/dead stains are also called viability dyes, and different types of dyes use different chemistry to stain live or dead cells. Consider these factors when including a live/dead stain in flow cytometry panels.Read the full article »
The word “validation” strikes fear and anxiety in the hearts of many researchers crossing into the world of flow cytometry for clinical trials. Validation plans evaluate assay reliability and overall performance and may include but are not limited to measuring precision, robustness, sample stability, assay specificity, and intersample variability.
Many research scientists working in basic research settings haven’t had to consider how to translate a basic flow cytometry protocol into a validated protocol and may think this is such an overwhelming process that they should avoid clinical trial research altogether. However, any well-trained scientist can develop a validated flow cytometry assay, especially with the assistance or collaboration with scientists who have validation expertise.Read the full article »