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Computational Cytometry | Flow Cytometry Data Analysis in the Era of Quantitative Data Science

News from FlowMetric

  • Preclinical Toxicology and Clinical Studies - Using Flow Cytometry throughout Development View Post Summary

    The pursuit of better medicine may take many years because of the complicated and often indirect path of biomedical research. Do you find yourself confused or unclear about when to use flow cytometry in “bench-to-bedside” research? The key is to understand the differences between preclinical and clinical research.

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  • Cytokine Release Syndrome and CAR-T cells: Challenges to Cutting-Edge Therapies View Post Summary

    Personalized cancer treatments, particularly chimeric antigen receptor (CAR) T cell therapy, have revolutionized the treatment of blood cancers and solid tumors. CAR T cell-based treatments use genetically modified T cells from a patient that are engineered to express a modified T cell receptor, which includes an extracellular binding domain that binds specifically to a patient’s tumor antigen, a transmembrane domain, and an intracellular domains that has multiple costimulatory domains (i.e., CD28, 4-1BB). Patients typically undergo lymphodepletion chemotherapy and are infused with their specific CAR -T cells, which are activated upon encounter with tumor antigen and can proliferate and lyse tumor cells. Although this type of treatment has been shown to be very effective for treating a wide range of tumors, severe side effects can occur, including cytokine release syndrome (CRS). Flow cytometry is a critical tool for understanding CRS and identifying biomarkers that may predict the onset of this complication.

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  • 3 Tips For Hybridoma Development View Post Summary

    Hybridoma Development to create custom monoclonal antibodies is a multiphase process and with all the new technologies available to the scientist, the overall process might be a little overwhelming. Although hybridoma development cannot really be described as a black art, there are several important steps that can really impact the outcome of your monoclonal antibody production and even though the process of PEG fusion is fairly straight forward, imposing a common sense approach can make a difference.

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