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News from FlowMetric

  • Five Fundamentals of Cell Sorting View Post Summary

    Fluorescence-activated cell sorting is a powerful tool for basic and clinical research because individual cells can be separated from a heterogeneous sample and used for downstream analysis or therapeutic applications. A fluorescent activated cell sorter works in a similar way as a flow cytometer. A single-cell suspension of fluorescently labeled cells pass through a fluidic system, and lasers excite the fluorescent molecules, which causes a change in the charge of the droplet containing the cell. This shift in charge is used to divert each droplet into a collection tube so relatively pure cell populations can be collected. Cell sorting can be done by any researcher, but many scientists work with contract research organizations that have expertise optimizing protocols for different yields or levels of purity.

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  • Top 3 Things To Consider When Developing A Clinical Flow Cytometry Assay View Post Summary

    Flow cytometry has been developed and used as a clinical tool since the invention of the first cytometers in the 1970s. At present, flow cytometry is considered essential for many routine clinical diagnostics, including assays for leukemia and lymphoma, stem cell enumeration, solid organ transplantation, HIV infection status, immunodeficiencies, and hematologic abnormalities. Many scientists involved in clinical trials or drug development are faced with developing clinical flow cytometry assays for multiple phases of clinical development.

    If you find yourself starting to plan a clinical flow cytometry assay, here are the top 3 issues to think about as you plan your experiment.

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  • Using Flow Cytometry in Preclinical Toxicology and Safety Assessment View Post Summary

    Drugs and biologics in the research pipeline must undergo stringent preclinical toxicology and safety assessments before use in a clinical trial. Most traditional preclinical toxicology screenings include testing in animal models in order to define toxicological and pharmacological parameters that are critical to determining appropriate dosing such as maximum tolerated dose (MTD).

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