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Tissue Dissociation Steps Video

 

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News from FlowMetric

  • Tips for Measuring T Cell Proliferation by Flow Cytometry View Post Summary

    Many basic and clinical immunology studies that focus on T cells include proliferation assays in order to determine if T cells are capable of proliferating under different in vitro or in vivo conditions. Flow cytometry is the ideal approach for measuring T cell proliferation and a suite of staining products now exist that allow you to incorporate a proliferation dye into your existing flow cytometry staining panel. As with all flow cytometry staining protocols, be sure to do a pilot experiment to titrate your staining reagents and determine how many cells you need to run during your flow cytometry acquisition to have enough events for an accurate proliferation measurement.

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  • Cross Reactive Antibodies for Flow Cytometry View Post Summary

    Flow cytometry is such a powerful and precise tool in part due to the vast number of antibodies available for use in staining panels. These antibodies can specifically recognize molecules on the surface of cells or within intracellular compartments. Most primary antibodies that are commercially available are specific to the main laboratory research species currently in use, including, mouse, rat, rabbit, pig, non-human primate, and human. But what do you do if you need to stain a molecule on a cell from a species not represented by these products? Many antibodies exhibit cross reactivity between species or subspecies, and online resources as well as pilot experiments can help you find an antibody that works for your particular sample.

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  • Mechanical versus Enzymatic Dissociation of Tissue for Flow Cytometry View Post Summary

    Flow cytometry is a powerful tool because it allows users to analyze the characteristics of millions of cells with relative speed and precision. A single cell suspension of fluorescently labeled sample travels through the cytometer for excitation by lasers, and the emitted light photons are measured by different detectors. Having a single cell suspension is essential to measuring cell fluorescence accurately, and many types of cell or tissue samples must be specially processed to make this suspension. Two different methods can be used for single cell suspensions: mechanical dissociation of tissue or enzymatic dissociation of tissue. This processing step is typically carried out before cells are stained and both methods have benefits and caveats.

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