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Tissue Dissociation Steps Video


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News from FlowMetric

  • In Search of Mesenchymal Stem Cells (MSCs) View Post Summary

    Mesenchymal stem cells (MSCs) are “hot” in biomedical research these days because they are multipotent stem cells that can be found in adipose (fat) tissue, umbilical cord blood and tissue, placenta, and bone marrow. MSCs behave like stem cells and, under specific conditions, can be induced to differentiate into specific cell types like adipocytes, chondrocytes or osteocytes. MSCs appear to be an exceedingly valuable potential source of stem cells for therapeutic use and are being studied in many diverse areas of biomedical research. One could imagine that in the future MSCs could be obtained from an individual, purified and induced to differentiate into a specific cell population and then be infused back into the same individual as a form of immunotherapy.

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  • T Cell Exhaustion – Using Flow Cytometry to Monitor this Immuno-Oncology Impediment View Post Summary

    T cells are well known for their roles in combating cancer and infection, but chronic exposure to antigens and inflammation can cause T cells to enter a state of “exhaustion[1].” Exhausted T cells lose critical effector functions including cytokine production, the ability to proliferate and memory T cell differentiation is also compromised. Exhausted T cells also express inhibitory receptors and become unresponsive to IL-7 and/or IL-15-driven self-renewal. This progression toward T cell exhaustion results in diminished control of chronic infection or cancer. Exhaustion can occur in both CD4+ and CD8+ T cell populations and the phenotypes of these subsets is somewhat heterogeneous. Nonetheless, T cell exhaustion is reversible and various immuno-oncology interventions have been examined or are currently being evaluated in order to improve outcomes in cancer and chronic infection[2].

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  • Method Match Up:  Automated Cell Counter vs Hemocytometer View Post Summary

    Every flow cytometry experiment, whether it begins in a culture flask or a mouse spleen, requires at least one step in which cells are counted. This step is essential to ensuring that you have a sufficient number of cells to evaluate by flow cytometry and that your staining and processing protocols are done correctly.

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