Immunophenotyping is a type of flow cytometry-based analysis in which cells are stained with fluorescently-conjugated antibodies for specific surface and intracellular molecules that define different immune cell populations. Immunophenotyping has been widely used for decades since the advent of cytometers that can detect four colors, and today’s state-of-the-art cytometers allow for concurrent immunophenotypic analysis of numerous cell subsets.
Immunophenotyping has been critical to immuno-oncology research because it allows researchers to monitor changes in the immune system, including potent anti-tumor responses or unexpected off-target responses.Read the full article »
Flow cytometry products have never been more widely available and varied. In fact, browsing flow cytometry product offerings may give an inexperienced user the impression that pre-existing staining kits will take care of all the work of developing a new assay. In contrast, experienced flow cytometry users may be more comfortable piecing together new staining panels on their own. Consider these benefits and challenges of using pre-existing staining kits compared with custom panels.Read the full article »
What is flow cytometry? Flow cytometry is a semi-quantitative technique that allows you to analyze the frequency and other properties of cells stained with specific fluorochrome-conjugated antibodies. Flow cytometry is most frequently used to monitor the immune response due to the fact that the frequency and functionality of different immune cell subsets can be measured concurrently. Flow cytometers are the instrument at the center of this technique and have evolved over the past few decades to become an essential instrument for biomedical research.Read the full article »