Many basic and clinical immunology studies that focus on T cells include proliferation assays in order to determine if T cells are capable of proliferating under different in vitro or in vivo conditions. Flow cytometry is the ideal approach for measuring T cell proliferation and a suite of staining products now exist that allow you to incorporate a proliferation dye into your existing flow cytometry staining panel. As with all flow cytometry staining protocols, be sure to do a pilot experiment to titrate your staining reagents and determine how many cells you need to run during your flow cytometry acquisition to have enough events for an accurate proliferation measurement.Read the full article »
The cell cycle is a defining characteristic of living organisms. In multicellular organisms, including humans, the cell cycle governs the duplication of cells in a manner that assures accurate DNA replication and cell division. Cell cycle regulation is considered such an essential element of biology that Leland H. Hartwell, R. Timothy Hunt and Paul M. Nurse were recognized with the 2001 Nobel Prize in Physiology for their discoveries of "key regulators of the cell cycle."Read the full article »
Cellular functions are what cells do to stay alive, proliferate, or defend themselves. Before the advent of flow cytometry, functional assays were done on multiple platforms and were labor intensive. In the last several years, flow cytometry-based functional assays have emerged as fast, precise and customizable assays that can be used in basic, preclinical or clinical research settings.
Check out these examples of flow cytometry-based functional assays that are currently in use across multiple therapeutic research areas.Read the full article »