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News from FlowMetric

  • Keeping Control of your Flow Cytometry Assay – FMOs and Isotype Controls View Post Summary

    High quality flow cytometry assay data can be obtained with well-maintained flow cytometers and high-quality processing and staining protocols. At the center of these operations, are the inclusion of the appropriate control samples, which is critical to guaranteeing the quality of flow cytometry assay data. Isotype controls and fluorescence minus one (FMO) controls are critical in assuring that the correct fluorescence signals are being measured, and also that these controls are separate from compensation controls which address spectral overlap effects. Consider these aspects of FMOs and isotype controls as you plan your next flow cytometry experiment.

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  • Cell Sorting for Monoclonal Antibody Production View Post Summary
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  • Five Facts about Phosphoflow Cytometry Assays View Post Summary

    Phosphoflow cytometry assays are becoming a valuable tool for researchers developing immuno-oncology applications because data from these assays can provide critical mechanistic insights. Phosphoflow assays measure phosphorylated proteins in cells, which is a critical readout for cell signaling responses. Check out these five facts about phosphoflow cytometry and consider adding this tool to your cytometry toolbox.

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