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News from FlowMetric

  • T Cell Exhaustion – Using Flow Cytometry to Monitor this Immuno-Oncology Impediment View Post Summary

    T cells are well known for their roles in combating cancer and infection, but chronic exposure to antigens and inflammation can cause T cells to enter a state of “exhaustion[1].” Exhausted T cells lose critical effector functions including cytokine production, the ability to proliferate and memory T cell differentiation is also compromised. Exhausted T cells also express inhibitory receptors and become unresponsive to IL-7 and/or IL-15-driven self-renewal. This progression toward T cell exhaustion results in diminished control of chronic infection or cancer. Exhaustion can occur in both CD4+ and CD8+ T cell populations and the phenotypes of these subsets is somewhat heterogeneous. Nonetheless, T cell exhaustion is reversible and various immuno-oncology interventions have been examined or are currently being evaluated in order to improve outcomes in cancer and chronic infection[2].

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  • Spooky Cytometry - Watch Out for Zombie Data and Dead Cells! View Post Summary

    Immunophenotyping is a commonly used flow cytometry method for discriminating between different cell subsets based on intracellular and cell surface markers. A critical parameter for running a successful immunophenotyping assay is to ensure that you are analyzing live cells. Dead cells are not useful for data analysis and can do strange things when stained.

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  • Q is for Quality - QA, QC and Flow Cytometry View Post Summary


    Q is for Quality - QA, QC and Flow Cytometry

    How do clinical flow cytometry labs ensure that the data they generate is accurate, reproducible, and conforms to regulatory requirements? They use quality management systems, including quality assurance (QA) and quality control (QC). Some scientists seem to use these terms interchangeably, but what do they really mean and why are they important to flow cytometry?

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