Memory is a characteristic of the immune system that provides humans and other vertebrates with long term protection against infectious diseases and other “non-self” antigens such as those associated with tumor cells. In the context of T cells, memory responses occur when a naive T cell encounters an antigen bound to a major histocompatibility complex molecule and is activated to undergo differentiation into an effector cell or a memory cell. Memory T cell populations can persist in the body for months to years and can be stimulated to respond specifically and rapidly to a foreign antigen upon re-exposure.Read the full article »
Flow cytometry assays are important for preclinical and clinical research, however, it is vital to understand the level of compliance required for the stage of research you are completing. Flow Cytometry assays completed for toxicology and safety assessments are required to be in compliance of Good Laboratory Practices (GLP), on the other hand, basic research or discovery/exploratory studies can be non-GLP. GLP refers to a set of standards for laboratory studies to be planned, performed, monitored, reported, and archived. Preclinical and clinical studies must be GLP-compliant in order to be submitted for review by regulatory agencies like the FDA. Consider these three points if you find yourself in need of a GLP-compliant flow cytometry assay.Read the full article »
Immune cell infiltration is essential for anti-tumor responses because immune cells must be able to be in close proximity to tumor cells for these responses to be effective. Flow cytometry is a flexible and powerful tool for detecting and monitoring the presence of infiltrating immune cells and can be customized to detect any immune cell subset.
Consider these questions as you think about assessing infiltrating immune cells with flow cytometry in your Immuno-Oncology (IO) research?Read the full article »