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The pursuit of better medicine may take many years because of the complicated and often indirect path of biomedical research. Do you find yourself confused or unclear about when to use flow cytometry in “bench-to-bedside” research? The key is to understand the differences between preclinical and clinical research.Read the full article »
In this era of doing more with less in the lab, you may be familiar with the many services CROs can provide to support researchers. CROs offer a wide array of research support from animal studies to assay development, but many scientists consider CROs as a short term arrangement to help with the occasional burdensome workload.
For long-term projects, a CRO can become a formidable research partner and can greatly enhance your lab’s technical capabilities and productivity. What should you consider if you want to transform your relationship with a CRO from being just a service provider to a research partner?Read the full article »
We walked you through the basics of achieving your optimal panel design in the “Know your Flow” series, but you probably have more questions as you refine your panel or stumble upon some of the technical nuances of staining cells.
Check out these tips for a successful flow cytometry run.
1. How Many Cells? Flow cytometry experiments “flow” best when you use a single cell suspension, and the ideal cellular density usually ranges from 1 X 105 to 1 X 106 cells /ml in 100 µl. How do you maintain your cells in a happy single-cell suspension state? You may need to strain cells during sample preparation. You will also typically use a solution of phosphate-buffered saline containing 1% bovine serum albumin or human serum throughout your staining protocol in order maintain cell viability and prevent cell clumping.Read the full article »