Tissue Dissociation Steps Video

 

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Immuno-Oncology Research and Flow Cytometry

News from FlowMetric

  • What Is Flow Cytometry? View Post Summary

    Flow cytometry is a semi-quantitative technique that allows you to analyze the frequency and other properties of cells stained with specific fluorochrome-conjugated antibodies. Flow cytometry is most frequently used to monitor the immune response due to the fact that the frequency and functionality of different immune cell subsets can be measured concurrently. Flow cytometers are the instrument at the center of this technique and have evolved over the past few decades to become an essential instrument for biomedical research.

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  • Sticky Situations - Using Cells from Tissue or Peripheral Whole Blood for Flow Cytometry [VIDEO] View Post Summary

    Flow cytometry is most often used to study cells from the immune system. These cells isolated often from blood in the peripheral circulation, but can also be harvested from tissues. Cells found in tissues or organs are usually tightly attached to these sites, and this is a fundamental property of these cells that allows them to surveil the immune system and launch responses. However, additional cell preparation steps must be used in order to dissociate these cells from the tissue so they can be used for flow cytometry. Consider these technical aspects of using cells from the circulation or tissue as you plan your next flow cytometry experiment.

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  • Measuring Memory - Evaluating Memory T Cells In A Clinical Setting View Post Summary

    Memory is a characteristic of the immune system that provides humans and other vertebrates with long term protection against infectious diseases and other “non-self” antigens such as those associated with tumor cells. In the context of T cells, memory responses occur when a naive T cell encounters an antigen bound to a major histocompatibility complex molecule and is activated to undergo differentiation into an effector cell or a memory cell. Memory T cell populations can persist in the body for months to years and can be stimulated to respond specifically and rapidly to a foreign antigen upon re-exposure.

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