Immune cell infiltration is essential for anti-tumor responses because immune cells must be able to be in close proximity to tumor cells for these responses to be effective. Flow cytometry is a flexible and powerful tool for detecting and monitoring the presence of infiltrating immune cells and can be customized to detect any immune cell subset.
Consider these questions as you think about assessing infiltrating immune cells with flow cytometry in your Immuno-Oncology (IO) research?Read the full article »
In several of our blogs, we have discussed the power of flow cytometry to identify unique cell populations, both rare and abundant. Flow cytometry also offers the opportunity to actually sort out the cells of interest for a variety of downstream applications. Fluorescence Activated Cell Sorting (FACS) and immunomagnetic cell sorting (MACS) are two of the most widely used methods for the isolation of phenotypically identified cells. Read more to make sense of FACS, MACS, and finding the best separation strategy for your needs.Read the full article »
Mesenchymal stem cells (MSCs) are “hot” in biomedical research these days because they are multipotent stem cells that can be found in adipose (fat) tissue, umbilical cord blood and tissue, placenta, and bone marrow. MSCs behave like stem cells and, under specific conditions, can be induced to differentiate into specific cell types like adipocytes, chondrocytes or osteocytes. MSCs appear to be an exceedingly valuable potential source of stem cells for therapeutic use and are being studied in many diverse areas of biomedical research. One could imagine that in the future MSCs could be obtained from an individual, purified and induced to differentiate into a specific cell population and then be infused back into the same individual as a form of immunotherapy.Read the full article »